a2780 ovarian carcinoma  (AddexBio Inc)

Journal: RSC Medicinal Chemistry

Article Title: meso -Substituted AB 3 -type phenothiazinyl porphyrins and their indium and zinc complexes photosensitising properties, cytotoxicity and phototoxicity on ovarian cancer cells †

doi: 10.1039/d4md00601a

Figure Lengend Snippet: (I) Fluorescence microscopy images of A2780 cells treated with porphyrin derivatives 2a, 4a, 5a, and 6a at a final concentration of 20 μM under standard cell culture conditions (37 °C, 5% CO 2 ), incubated for 24 (top) and 72 hours (bottom). (II) Fluorescence microscopy images of A2780 cells after 72 hours treatment with 20 μM derivatives 2a, 4a, 5a, 6a, and an untreated control, in comparison with their corresponding bright field images.

Article Snippet: We performed an in vitro testing on A2780 ovarian carcinoma cell line, acquired from the European Collection of Authenticated Cell Cultures (ECACC, Salisbury, Great Britain).

Techniques: Fluorescence, Microscopy, Concentration Assay, Cell Culture, Incubation, Control, Comparison

Journal: RSC Medicinal Chemistry

Article Title: meso -Substituted AB 3 -type phenothiazinyl porphyrins and their indium and zinc complexes photosensitising properties, cytotoxicity and phototoxicity on ovarian cancer cells †

doi: 10.1039/d4md00601a

Figure Lengend Snippet: Variations of metabolic activity in A2780 cells treated with sublethal concentration of 2, 2a, 4, 4a,b, 5, 5a, 6a and TPP 6 in dark, and photoirradiated abbreviated PDT (LED irradiation λ = 505 nm, time 150 s), respectively.

Article Snippet: We performed an in vitro testing on A2780 ovarian carcinoma cell line, acquired from the European Collection of Authenticated Cell Cultures (ECACC, Salisbury, Great Britain).

Techniques: Activity Assay, Concentration Assay, Irradiation

Journal: RSC Medicinal Chemistry

Article Title: meso -Substituted AB 3 -type phenothiazinyl porphyrins and their indium and zinc complexes photosensitising properties, cytotoxicity and phototoxicity on ovarian cancer cells †

doi: 10.1039/d4md00601a

Figure Lengend Snippet: The metabolic rate evolution of treated A2780 cells, within 72 hours interval, in the presence or absence of photoirradiation (PDT). Compounds 2a, 4a, 5a and 6a were assessed at the concentrations of 5; 15; 25 and 50 μM, and the measurements were made at 24, 48 and 72 hours.

Article Snippet: We performed an in vitro testing on A2780 ovarian carcinoma cell line, acquired from the European Collection of Authenticated Cell Cultures (ECACC, Salisbury, Great Britain).

Techniques:

Journal: RSC Medicinal Chemistry

Article Title: meso -Substituted AB 3 -type phenothiazinyl porphyrins and their indium and zinc complexes photosensitising properties, cytotoxicity and phototoxicity on ovarian cancer cells †

doi: 10.1039/d4md00601a

Figure Lengend Snippet: Quantitative evaluation of intracellular Nrf2 modulation following the A2780 cell populations treatment with the compounds 2, 2a, 4, 4a, 4b, 5, 5a, 6 and 6a in absence (abbreviated as 2 to 6a) or presence of photoirradiation (abbreviated as 2 -PDT to 6a -PDT ). Significant reduction of Nrf2 expression were highlighted with red starring: 5 versus the reference untreated cells, and respectively 4a -PDT and 6 -PDT versus untreated irradiated cells.

Article Snippet: We performed an in vitro testing on A2780 ovarian carcinoma cell line, acquired from the European Collection of Authenticated Cell Cultures (ECACC, Salisbury, Great Britain).

Techniques: Expressing, Irradiation

Journal: RSC Medicinal Chemistry

Article Title: meso -Substituted AB 3 -type phenothiazinyl porphyrins and their indium and zinc complexes photosensitising properties, cytotoxicity and phototoxicity on ovarian cancer cells †

doi: 10.1039/d4md00601a

Figure Lengend Snippet: The reactive oxygen species (ROS) level in A2780 cells treated with 20 μM 2, 2a, 4, 4a,b, 5, 5a, 6a or TPP 6 in dark conditions versus light irradiation (abbreviated PDT), as indicated by the fluorescence intensity of the samples. The following abbreviations were used: 2, 2a, 4, 4a,b, 5, 5a, 6, 6a correspond to the A2780 cells treated with the compounds, TPP 6 is the reference tetraphenylporphyrin, C the untreated control, C-PDT the untreated and photoirradiated control while 2 -PDT , 2a -PDT , 4 -PDT , 4a -PDT , 4b -PDT , 5 -PDT , 5a -PDT , 6 -PDT , 6a- PDT is the code for the samples treated and photoirradiated. The starred columns represent statistically significant increases in ROS production.

Article Snippet: We performed an in vitro testing on A2780 ovarian carcinoma cell line, acquired from the European Collection of Authenticated Cell Cultures (ECACC, Salisbury, Great Britain).

Techniques: Irradiation, Fluorescence, Control

Journal: RSC Medicinal Chemistry

Article Title: meso -Substituted AB 3 -type phenothiazinyl porphyrins and their indium and zinc complexes photosensitising properties, cytotoxicity and phototoxicity on ovarian cancer cells †

doi: 10.1039/d4md00601a

Figure Lengend Snippet: The level of TNF-α secreted by A2780 ovarian tumour cells, following the treatment with the compounds 2, 2a, 4, 4a, 4b, 5, 5a, 6 and 6a in absence (abbreviated as 2 to 6a) or presence of photoirradiation (abbreviated as 2 -PDT to 6a -PDT ). The starred columns indicate significant increase or decrease of extracellular TNF-α, induced by compounds and irradiation, within 24 hours.

Article Snippet: We performed an in vitro testing on A2780 ovarian carcinoma cell line, acquired from the European Collection of Authenticated Cell Cultures (ECACC, Salisbury, Great Britain).

Techniques: Irradiation

Journal: Journal of Natural Products

Article Title: Undescribed Amaryllidaceae Alkaloids from Zephyranthes citrina and Their Cytotoxicity

doi: 10.1021/acs.jnatprod.4c00825

Figure Lengend Snippet: Cytotoxicity Screening of Alkaloids Isolated from Z. citrina Baker a

Article Snippet: Selected human tumor and nontumor cell lines Jurkat (acute T-cell leukemia), MOLT-4 (acute lymphoblastic leukemia), A549 (lung carcinoma), HT-29 (colorectal adenocarcinoma), PANC-1 (pancreas epithelioid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma), MCF-7 (breast adenocarcinoma), SAOS-2 (osteosarcoma) and MRC-5 (nontumor lung fibroblasts) were purchased from European Collection of Authenticated Cell Cultures (ECACC, Salisbury, UK) and cultured according to the provider’s culture method guidelines.

Techniques: Isolation